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Two-way ANOVA
The two-way analysis of variance is an extension to the one-way analysis of variance. There are two independent variables (hence the name two-way). Two-way Analysis of Variance is a way of studying the effects of two factors separately (their main effects) and (sometimes) together (their interaction effect).
Assumptions
- The populations from which the samples were obtained must be normally or approximately normally distributed.
- The samples must be independent.
- The variances of the populations must be equal.
- The groups must have the same sample size.
Hypotheses
There are three sets of hypothesis with the two-way ANOVA.
The null hypotheses for each of the sets are given below.
- The population means of the first factor are equal. This is like the one-way ANOVA for the row factor.
- The population means of the second factor are equal. This is like the one-way ANOVA for the column factor.
- There is no interaction between the two factors. This is similar to performing a test for independence with contingency tables.
Factors
The two independent variables in a two-way ANOVA are called factors. The idea is that there are two variables, factors, which affect the dependent variable. Each factor will have two or more levels within it, and the degrees of freedom for each factor is one less than the number of levels.
Treatment Groups
Treatement Groups are formed by making all possible combinations of the two factors. For example, if the first factor has 3 levels and the second factor has 2 levels, then there will be 3x2=6 different treatment groups.


